We investigated frictional sliding behavior of mixture gouges of quartz with various weight proportions of biotite and their structured equivalents with same weight proportions of biotite as layers embedded in quartz gouge. Our experiments were performed under effective confining pressure of 200MPa, pore pressure of 30MPa, temperature of 100℃ and the shear displacement rate of 1.22μm/s. The results show that for structured gouges with biotite layers embedded in quartz gouge as a weak structure, the strength has a power law decreasing trend with increasing weight proportions of biotite. The fault gouges can be weakened significantly by as little as 5wt% biotite, and 30wt% biotite corresponds to a beginning point of less sensitive strength change in response to increasing biotite proportion. On the other hand, the strength of mixed gouges shows a linear decreasing trend with increasing biotite proportion. Microstructures of deformed samples show that in mixed gouges, biotite and quartz are both sheared and grain size extremely reduced, and their contributions to overall strength have a close relation with their respective contents. However, in structured gouges, the shear deformation mainly occurred in the weak biotite layers with no shears crossing the quartz gouge. These results confirm the importance of the weak fabric in its effect on frictional strength. If the weak minerals form foliations and interconnected arrangements, it will lead to weakness of fault zones.